Where to find planaria
Planarians are interesting to study for a variety of reasons. First, planarians have bilateral symmetry with two nerves extending the length of the body, an enlarged "brain" ganglion cell , and two eye spots.
The eye spots are sensitive to light. Planarians move away from the light and are most active in the dark. Second, light and water current can be used demonstrate kinesis change of speed and taxis change of direction. Teach long term earth changes in real time and study the atmosphere, weather and climate and their impact on sustaining life. One stop for all your classical mechanics science and energy education needs. Exciting activities that make science active and fun!
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The planaria will arrive packaged in a small jar or spring water. Remove and inspect the jar. Unscrew the lid and rest it on top of the jar to allow air exchange that is vital to planarian survival. Planarians foul their water rapidly.
As long as the water in the jar remains clear and you plan to use the cultures that day or the next, little additional care is needed. If you plan to keep the planaria longer than a day or two, follow the additional care information below. If the water in the shipping jar is at all cloudy, transfer the organisms to fresh, natural spring water immediately.
Planaria are not suitable for long term culturing, but, with diligence, a culture can be maintained for several weeks. To do this, you will need a housing container, a food source and natural spring water.
We do not recommend using tap water, but if no other water source is available, condition your tap water using a water conditioner. You will need an 8" culture dish or another shallow, glass, plastic, enameled or stainless steel container. We recommend covering your container or providing hiding places, as planaria will naturally avoid light. You will also need a source of natural spring water.
Planaria need to have their water changed daily. Pour 2 to 5 cm of fresh spring water into the container. Use a dropping pipette to move the planarians.
It may be necessary to carefully dislodge some individuals with your finger. Feed planarians once a week. Suitable foods include fresh beef liver, hard-boiled egg yolk, Lumbriculus , pieces of earthworm, crushed aquarium snails, etc.
For up to 50 planarians, feed a pea-sized portion. After 30 minutes, transfer the planaria to a fresh container of spring water. Carolina provides living organisms for educational purposes only. As a general policy, we do not advocate the release of organisms into the environment.
In some states, it is illegal to release organisms, even indigenous species, without a permit. The intention of these laws is to protect native wildlife and the environment. Tap water often contains metal ions that are detrimental to planarians. During their sexual period, generally February or March, black and brown planarians are fragile. Do not handle or feed them during this time.
They may deposit cocoons on the bottom of the culture dish. If maintained in fresh spring water, the cocoons will hatch in 2 to 3 weeks, giving rise to several small planaria. The anterior end of the planarian is more sensitive to toxins; if a toxic substance is in the water, the anterior end will degenerate first.
Use spring water, not tap water. If you are using spring water from a grocery store, there may be a problem with it. Locally collected spring or pond water may contain a pollutant, or your containers may have soap or detergent residue in them. Which planarians should I use for regeneration experiments? Black and brown planaria are your best choice. It will take them about 2 weeks to regenerate at room temperatures. White planaria will regenerate, but they take longer.
It is unknown whether a September discovery in an outdoor habitat in Massachusetts indicates an established population or a temporary one that will die off during the winter L. Novak, personal communication. The widespread occurrence of land planarians is a result of horticultural practices and dispersion of potted plants in commerce.
In tropical and subtropical areas, once planarians are established in a greenhouse they disperse to the adjacent environment. Land planarians are soft, bilaterally symmetric, acoelomate, dorsally-ventrally flattened worms, 3 to 50 cm long by 0. They lack a respiratory and circulatory system, a skeleton, and an anus. The heads of many land planarians are expanded lunate or tapering to a blunt point. Eyespots may be present on the head.
Colors of Florida species range from greenish-gray to brown with dark narrow stripes on the dorsal side. A mouth, which also serves as an anus, is present near the mid-body on the ventral surface. A protrusible muscular plicate pharynx serves as a feeding organ and is attached to a three-branched intestine.
The space between organs is filled with parenchyma. Circular and longitudinal muscles are present. A cerebral ganglion serves as a brain, innervating a ladder-shaped nervous system. Excretion of fluid wastes is accomplished with a primitive proto-nephridial system Esser Hyman described Dolichoplana striata Moseley. Form very elongated and flattened.
Ground color yellowish brown with 6 longitudinal dark stripes, paired median, lateral, and marginal. Median stripes are very narrow and may be absent in young specimens. Lateral stripes are very conspicuous, black, sharply defined, beginning at level of eyes and continuing to posterior tip. Marginal stripes are less dark, diffuse, ill defined, fading away posteriorly. Creeping sole white, bordered on each side by diffuse pigmentation. Bipalium kewense is recognized by its pale color, dorsal dark median line, dark patch in neck region, and expanded lunate head.
This species may attain lengths up to 30 cm. Chandler Reproduction and Development: Reproduction is principally by fragmentation at the posterior end. Lateral margins pinch in about 1 cm from the tail tip.
Severance occurs when the posterior fragment adheres to the substrate and the parent worm pulls away. The posterior fragment is immediately motile, and within seven to 10 days a lightly pigmented head begins to form.
One to two fragments are released each month. Bright red eggs are deposited in 0. Within 24 hours the cocoons turn black. Planarians emerge in approximately 21 days. Habitat: Because land planarians are photo-negative during daylight hours and require high humidity, they are found in dark, cool, moist areas under objects such as rocks, logs, in debris, or under shrubs, and on the soil surface following heavy rains.
Land planarians are also found in caves, but are rare in rural sites. Movement and feeding occur at night. High humidity is essential to survival. They can survive desiccation only if water loss does not exceed 45 percent of their body weight.
Land planarians are most abundant in spring and fall. Dundee and Dundee reported Bipalium kewense as being plentiful enough in New Orleans to be used as demonstration material in zoology classes. Locomotion: Land planarians glide smoothly on the substrate by the action of powerful, closely spaced cilia in a special medial ventral strip creeping sole , on a thin coat of mucus secreted on the substrate by glands opening into the creeping sole.
Land planarians that migrate on plants or objects sometimes regain the ground by lowering themselves down by a string of mucus. Nutrition: Land planarians devour earthworms, slugs, insect larvae, and are cannibalistic. Prey are located by chemoreceptors located in a single ciliated pit under the head or in a ciliated ventral groove. Struggling prey are held to the substrate and entangled in slimy secretions from the planarian.
The pharynx is protruded from the mouth and into the prey. Food is reduced to small particles prior to entering the gastrovascular cavity. The food particles are taken up by epithelial cells in amoeboid fashion and formed into food vacuoles. Planarians store food in digestive epithelium and can survive many weeks by shrinking slowly in size without feeding. They can use their own tissues, such as reproductive tissue, for food when reserves are exhausted.
Figure 3. Planarian A. Illustration from Esser Planarian Enemies: Other animals rarely devour land planarians, since surface secretions appear distasteful, if not toxic.
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